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蛋白质亚单位疫苗含有从病毒或细菌病原体中分离出的特定蛋白质；多糖疫苗含有在某些细菌的细胞壁中发现的糖分子链（多糖）；结合亚单位疫苗将多糖链与载体蛋白结合，以尝试增强免疫反应。亚单位疫苗已经被广泛使用，例子包括乙型肝炎和无细胞百日咳疫苗（蛋白质亚基）、肺炎球菌多糖疫苗（多糖）。",{"cn":272,"en":279,"jp":280,"kr":281},270,1090,786,406,1741094718,1743248061,{"id":286,"cateId":258,"catePath":9,"title":287,"lang":11,"path":288,"mainPic":289,"remark":9,"content":290,"otherLangId":292,"otherLangPath":20,"otherLangTitle":20,"cateName":9,"status":21,"sort":21,"pv":296,"statusText":9,"address":9,"city":9,"positionType":9,"recruitType":9,"employmentType":9,"createTime":297,"updateTime":298,"startTime":21,"stopTime":21,"breadCrumbs":20},32,"病毒样颗粒","bing-du-yang-ke-li","/dnweb/c2b28af0e3009131466c3c0fdcddef45.png",{"desc":291,"pics":289,"url":9},"大多数当前可用的疫苗主要基于灭活或减毒活疫苗。尽管这些传统疫苗已被有效地用于对抗各种传染病，但其中一些有一些局限性，包括它们诱导更强免疫反应的可能性较低和疗效不佳。最近爆发的传染病表明需要开发强大的疫苗来克服这些限制。主要挑战是开发新的技术方法，并在不损害安全性、有效性和耐受性的情况下，增强免疫力。基于 DNA、mRNA 和重组病毒载体的疫苗的最新进展为难以靶向的病原体和控制传染病爆发提供了有效的疫苗开发方法。",{"cn":286,"en":293,"jp":294,"kr":295},269,1089,785,210,1741094690,1743248218,{"id":300,"cateId":258,"catePath":9,"title":301,"lang":11,"path":302,"mainPic":303,"remark":9,"content":304,"otherLangId":306,"otherLangPath":20,"otherLangTitle":20,"cateName":9,"status":21,"sort":21,"pv":310,"statusText":9,"address":9,"city":9,"positionType":9,"recruitType":9,"employmentType":9,"createTime":311,"updateTime":312,"startTime":21,"stopTime":21,"breadCrumbs":20},31,"DNA 疫苗","dna-yi-miao","/dnweb/83445ef4e57e1070a1e8410823cdac4d.png",{"desc":305,"pics":303,"url":9},"基于核酸的诊断、预后和治疗平台是很有前途的工具，由于其独特的特性，如热稳定性、抗变性和简单存储，它们正在取代旧的基于蛋白质的平台。一个好的疫苗平台应该是快速的、简单开发的、可再现的、热稳定的，并且可在降低开发成本和风险的情况下制造。 DNA 平台解决了其中的许多目标。",{"cn":300,"en":307,"jp":308,"kr":309},268,1088,784,214,1741094659,1743248406,{"id":314,"cateId":258,"catePath":9,"title":315,"lang":11,"path":316,"mainPic":317,"remark":9,"content":318,"otherLangId":320,"otherLangPath":20,"otherLangTitle":20,"cateName":9,"status":21,"sort":21,"pv":324,"statusText":9,"address":9,"city":9,"positionType":9,"recruitType":9,"employmentType":9,"createTime":325,"updateTime":326,"startTime":21,"stopTime":21,"breadCrumbs":20},30,"mRNA 疫苗","mrna-yi-miao","/dnweb/b7b2f858359af3f3242d59379f1bc652.png",{"desc":319,"pics":317,"url":9},"早在针对 SARS-CoV-2 的 mRNA 疫苗将这项技术推向舞台中央之前，mRNA 就已被确定为是一种潜在的抗肿瘤疫苗接种方法，而现在，鉴于COVID-19疫苗的巨大成功，mRNA已被探索用于其它各种领域，包括针对其它传染病的疫苗、抗肿瘤疫苗以及蛋白质补充/替代疗法等。尽管mRNA对几乎无处不在的核糖核酸酶敏感并且可能会快速降解，但其同样具有强大的安全性优势，非复制意味着它不与基因组相互作用，而其相对不稳定性和体内快速代谢清除可以通过使用各种修饰来调节。现有的成功经验已经证实，将mRNA制剂到不同的载体结构中后，可实现给药后的快速摄取和高效蛋白质表达。",{"cn":314,"en":321,"jp":322,"kr":323},236,1082,778,519,1741094134,1743249151,[328,343,356],{"id":329,"cateId":330,"catePath":9,"title":331,"lang":11,"path":332,"mainPic":333,"remark":9,"content":334,"otherLangId":336,"otherLangPath":20,"otherLangTitle":20,"cateName":9,"status":21,"sort":21,"pv":340,"statusText":9,"address":9,"city":9,"positionType":9,"recruitType":9,"employmentType":9,"createTime":341,"updateTime":342,"startTime":21,"stopTime":21,"breadCrumbs":20},60,49,"单克隆抗体 （mAb）","dan-ke-long-kang-ti-mab","/dnweb/9ff27d3a8bed341f4533b1cd243e0f6d.png",{"desc":335,"pics":333,"url":9},"单克隆抗体 (mAb) 是当今最成功的一类生物药，在过去的 20 年里，这类疗法发展迅速，如今有数百种 mAb 处于临床开发阶段，并且有众多的单克隆抗体产品已获准用于多种适应症，包括肿瘤、自身免疫性疾病以及罕见病适应症。",{"cn":329,"en":337,"jp":338,"kr":339},274,1094,790,299,1741417935,1743246798,{"id":344,"cateId":330,"catePath":9,"title":345,"lang":11,"path":346,"mainPic":347,"remark":9,"content":348,"otherLangId":350,"otherLangPath":20,"otherLangTitle":20,"cateName":9,"status":21,"sort":21,"pv":293,"statusText":9,"address":9,"city":9,"positionType":9,"recruitType":9,"employmentType":9,"createTime":354,"updateTime":355,"startTime":21,"stopTime":21,"breadCrumbs":20},59,"双特异性抗体（bsAb）","shuang-te-yi-xing-kang-ti-bsab","/dnweb/ee12a6c9e678d1e94f6a3704c0c0802f.png",{"desc":349,"pics":347,"url":9},"双特异性抗体（bsAb）可以同时结合两种不同类型的抗原或同一抗原上的两种不同表位，其可以按多种结构形式制备，大体可分为 IgG 样和非 IgG 样两大类，前者保留了传统mAb两个 Fab 臂和一个 Fc 区的结构，但两个 Fab 位点结合不同的抗原，其通常用 quadroma， 或杂合杂交瘤，方法制造，但这种方法依赖随机机会形成可用的 bsAb，效率低下。",{"cn":344,"en":351,"jp":352,"kr":353},273,1093,789,1741417844,1743247228,{"id":357,"cateId":330,"catePath":9,"title":358,"lang":11,"path":359,"mainPic":360,"remark":9,"content":361,"otherLangId":363,"otherLangPath":20,"otherLangTitle":20,"cateName":9,"status":21,"sort":21,"pv":367,"statusText":9,"address":9,"city":9,"positionType":9,"recruitType":9,"employmentType":9,"createTime":368,"updateTime":369,"startTime":21,"stopTime":21,"breadCrumbs":20},58,"抗体偶联药物（ADC）","kang-ti-ou-lian-yao-wu-adc","/dnweb/8974221c75bca598221dc792e5782c30.png",{"desc":362,"pics":360,"url":9},"抗体偶联药物（ADC）是由与生物活性细胞毒性有效载荷连接的抗体组成的复杂分子。ADC 结合了抗体的靶向能力以及细胞毒性药物的肿瘤杀伤能力。抗体将自身附着在肿瘤细胞表面的抗原上。抗体与目标蛋白（抗原）之间的生化反应在肿瘤细胞中触发信号，然后肿瘤细胞将抗体和连接的细胞毒素一起吸收或内化。内化后，细胞毒素会杀死肿瘤。这种靶向功能限制了副作用，并提供了比其它化学治疗药物更宽的治疗窗口。",{"cn":357,"en":364,"jp":365,"kr":366},272,1092,788,581,1741417552,1743247450,[371,386,399,413,426],{"id":372,"cateId":373,"catePath":9,"title":374,"lang":11,"path":375,"mainPic":376,"remark":9,"content":377,"otherLangId":379,"otherLangPath":20,"otherLangTitle":20,"cateName":9,"status":21,"sort":21,"pv":383,"statusText":9,"address":9,"city":9,"positionType":9,"recruitType":9,"employmentType":9,"createTime":384,"updateTime":385,"startTime":21,"stopTime":21,"breadCrumbs":20},54,50,"慢病毒","man-bing-du","/dnweb/a55344f507232973b696ba7adcfb5c57.png",{"desc":378,"pics":376,"url":9},"慢病毒是逆转录病毒的一种，粒径为80-120nm，由单链RNA序列组成，其转录成DNA并可整合到宿主基因组中，导致持续感染。大多数慢病毒载体源于HIV-1，并保留整合至感染细胞基因组的能力。LV由于能够更有效地转导非增殖或缓慢增殖的细胞，在临床应用中非常流行。近来，多个使用临床试验使用第三代自灭活LV将基因导入造血干细胞，以治疗原发性免疫缺陷或血红蛋白病[2]。LV最常见的应用是通过嵌合抗原受体（CAR）或克隆的T细胞受体递送引入基因，以产生对抗肿瘤的免疫。\n\n对于慢病毒的上游生产，尽管已经有研究证明制备稳定的慢病毒载体包装/生产细胞系是可行的，其有潜力提高批次间的一致性，保证供应并降低成本[6]，因其可简化LV生产的操作和供应链，但往往需要较长的细胞系开发构建时间，所以基于HEK293T细胞以质粒DNA瞬时转染的策略仍是慢病毒载体大规模生产的主要选择。对于细胞培养，在开发阶段，鉴于仅需要较小的批次体积，以及HEK293T细胞的贴壁特性和耗材的简单可获得性，以贴壁培养为主导。但随着规模放大，需要更高且更一致的批次规模，生产需转移至在摇摆式生物反应器或搅拌罐生物反应器中进行的悬浮培养，而培养基和添加物的选择直接与生产细胞的扩增和活性以及下游工艺的成功有关，因培养基可影响载体的稳定性并组成进入下游的进样料液，需仔细优化。\n\n相比AV或AAV，LV下游工艺的更大挑战在于其为囊膜病毒，固有的复杂性和敏感性需要快速的工艺处理，比如其对冻融循环、盐、pH、剪切以及缓冲液渗透压更为敏感，且颗粒热稳定性较低，37℃下半衰期为7-8 hr。LV的下游工艺也可分为3个主要阶段，包括收获和澄清；中间体纯化，其中包括1个或多个浓缩和纯化步骤；精纯、制剂以及选择性进行的除菌过滤。\n\nLV是细胞外产物，由生产细胞释放到培养液中，所以，收获液质量高度取决于细胞活性，其决定了宿主细胞源性杂质的含量。澄清旨在去除细胞和细胞碎片，深层过滤是该步骤一个简单且经济的选择，经优化后，可达到接近100%的收率。为提高最终产品的整体生物安全性，并符合法规标准，通常需要核酸酶处理，以消解核酸，这也有利于降低料液粘度，方便后续步骤操作，其通常在澄清步骤后进行，但也有报导在更为后续的阶段体积进一步降低后进行。\n\n层析是纯化方案的关键技术，旨在去除蛋白质、核酸、低分子量杂质以及脂质等，以结合-洗脱模式操作的阴离子交换层析和以流穿模式操作的复合模式层析已被报道单独或结合用于LV的纯化，但如前所述，由于LV对盐和pH等条件敏感，需仔细优化层析缓冲液条件。此外也有报道使用固定金属亲和层析和体积排阻层析，但通常限于较小的规模。TFF是快速且稳健的料液浓缩和换液技术，对于LV，可选择MWCO 750kD的中空纤维过滤器。药物底物灌装前的除菌过滤可使用0.22μm过滤器，但考虑LV较大的粒径，报道该步骤损失可达到30-50%，一种降低损失的策略是在最终TFF步骤之前进行除菌过滤，或者使用封闭系统实现无菌工艺而跳过该步骤。",{"cn":372,"en":380,"jp":381,"kr":382},279,1099,795,302,1741416315,1742879424,{"id":387,"cateId":373,"catePath":9,"title":388,"lang":11,"path":389,"mainPic":390,"remark":9,"content":391,"otherLangId":393,"otherLangPath":20,"otherLangTitle":20,"cateName":9,"status":21,"sort":21,"pv":201,"statusText":9,"address":9,"city":9,"positionType":9,"recruitType":9,"employmentType":9,"createTime":397,"updateTime":398,"startTime":21,"stopTime":21,"breadCrumbs":20},53,"溶瘤病毒","rong-liu-bing-du","/dnweb/cccd793c0b57b671db1b0319f75e8af6.png",{"desc":392,"pics":390,"url":9},"溶瘤病毒 (OV) 之所以被认为特别有前途，是因为它们具有两种主要的作用模式：它们既能杀死受感染的肿瘤细胞，又能刺激抗肿瘤免疫，以促进对未感染肿瘤细胞的杀伤。此外，还有其它连带的辅助作用。正因为如此，它们提供了极大增强现有肿瘤疗法的可能性。但与溶瘤病毒疗法相关的商业成功历史相对较短，尽管早前有一些基于腺病毒的溶瘤病毒疗法获得了中国和其它国家监管机构的批准，但第一个获得 FDA和EMA 批准并引起行业对此类疗法更大范围关注的溶瘤药物是在 2015 年，即Amgen的Talimogene laherparepvec (T-VEC) - 一种重组 HSV-1 溶瘤病毒，而最近获得批准的是来自Daiichi Sankyo的teserpaturev或G47Δ (DELYTACT®)，也是一种改良型HSV-1，于2021年6月在日本获得了用于恶性胶质瘤的有条件和有时限批准。",{"cn":387,"en":394,"jp":395,"kr":396},278,1098,794,1741416069,1743249666,{"id":400,"cateId":373,"catePath":9,"title":401,"lang":11,"path":402,"mainPic":403,"remark":9,"content":404,"otherLangId":406,"otherLangPath":20,"otherLangTitle":20,"cateName":9,"status":21,"sort":21,"pv":410,"statusText":9,"address":9,"city":9,"positionType":9,"recruitType":9,"employmentType":9,"createTime":411,"updateTime":412,"startTime":21,"stopTime":21,"breadCrumbs":20},52,"腺相关病毒","xian-xiang-guan-bing-du","/dnweb/3550971f826b58b9ad98cd3b2b480b4b.png",{"desc":405,"pics":403,"url":9},"腺相关病毒（AAV）是基因治疗中最常用的载体，其是非致病性细小病毒，直径约20 nm，无囊膜，基因组长约4.8 kb，其两端为反向重复序列（ITR），对复制、衣壳化等过程有重要作用，中间为rep与cap两个开放阅读框。",{"cn":400,"en":407,"jp":408,"kr":409},277,1097,793,208,1741415529,1742880786,{"id":373,"cateId":373,"catePath":9,"title":414,"lang":11,"path":415,"mainPic":416,"remark":9,"content":417,"otherLangId":419,"otherLangPath":20,"otherLangTitle":20,"cateName":9,"status":21,"sort":21,"pv":423,"statusText":9,"address":9,"city":9,"positionType":9,"recruitType":9,"employmentType":9,"createTime":424,"updateTime":425,"startTime":21,"stopTime":21,"breadCrumbs":20},"质粒 DNA","zhi-li-dna","/dnweb/7d8d88637a314eed94fe44c85eb9ab53.png",{"desc":418,"pics":416,"url":9},"质粒是存在于古生菌、细菌以及部分酵母中的含有染色体外遗传信息的小型双链环状 DNA 分子，其包含至少一个复制起点，可独立自主复制，一个细胞可以包含单个质粒的多个拷贝。质粒骨架序列的遗传信息可能不是必需的，但至少有助于细胞在其给定环境中生存。",{"cn":373,"en":420,"jp":421,"kr":422},276,1096,792,518,1741409058,1742878569,{"id":226,"cateId":373,"catePath":9,"title":427,"lang":11,"path":428,"mainPic":429,"remark":9,"content":430,"otherLangId":432,"otherLangPath":20,"otherLangTitle":20,"cateName":9,"status":21,"sort":21,"pv":436,"statusText":9,"address":9,"city":9,"positionType":9,"recruitType":9,"employmentType":9,"createTime":437,"updateTime":438,"startTime":21,"stopTime":21,"breadCrumbs":20},"外泌体","wai-mi-ti","/dnweb/95a9fbc511127d109566fa03e9993d86.png",{"desc":431,"pics":429,"url":9},"外泌体是细胞外囊泡的一种亚型，后者是源自细胞的脂质双层闭合结构，几乎由所有类型的细胞分泌，包括外泌体（30-150 nm）、微泡（150 nm 至 1 μm）和凋亡小体（1-5 μm）。",{"cn":226,"en":433,"jp":434,"kr":435},275,1095,791,333,1741408364,1742878558,[440,455,469],{"id":441,"cateId":442,"catePath":9,"title":443,"lang":11,"path":444,"mainPic":445,"remark":9,"content":446,"otherLangId":448,"otherLangPath":20,"otherLangTitle":20,"cateName":9,"status":21,"sort":21,"pv":452,"statusText":9,"address":9,"city":9,"positionType":9,"recruitType":9,"employmentType":9,"createTime":453,"updateTime":454,"startTime":21,"stopTime":21,"breadCrumbs":20},624,51,"肿瘤浸润淋巴细胞（TIL）","zhong-liu-jin-run-lin-ba-xi-bao-til","/dnweb/898b900c3b8f27777e788dd3c47c04c1.png",{"desc":447,"pics":445,"url":9},"肿瘤浸润淋巴细胞 (TIL) 是浸润实体瘤微环境 (TME) 的天然单核细胞，也可能称为肿瘤部位的所有免疫细胞。TIL 的历史可以追溯到两个多世纪前。1863 年，Virchow 观察到肿瘤组织中含有白细胞。1982 年，过继性细胞疗法 (ACT) 之父 Steven Rosenberg 首次从小鼠肿瘤模型中分离出 TIL，并报告称，联合使用环磷酰胺、TIL 和白细胞介素 (IL)-2 可以改善 50-100% 患有结肠腺癌且伴有肝或肺转移的小鼠。该报告支持了 TIL 疗法治疗晚期癌症的效果。他的团队也在 1988 年对人类进行了首次 TIL 疗法，使转移性黑色素瘤消退了 60%。",{"cn":441,"en":449,"jp":450,"kr":451},636,1292,988,561,1743597243,1747141053,{"id":456,"cateId":442,"catePath":9,"title":457,"lang":11,"path":458,"mainPic":459,"remark":9,"content":460,"otherLangId":462,"otherLangPath":20,"otherLangTitle":20,"cateName":9,"status":21,"sort":21,"pv":466,"statusText":9,"address":9,"city":9,"positionType":9,"recruitType":9,"employmentType":9,"createTime":467,"updateTime":468,"startTime":21,"stopTime":21,"breadCrumbs":20},623,"间充质干细胞（MSC）","jian-chong-zhi-gan-xi-bao-msc","/dnweb/ecd5eaceeecd050c5ae4d4e4cb6e217b.png",{"desc":461,"pics":459,"url":9},"从不同的组织中分离出来的间充质干细胞（MSC）代表了一个很大的临床研究领域。除了具有增殖和分化能力外，MSC还可以通过细胞间的直接接触、细胞因子的释放以及细胞外囊泡 (EV) 的分泌来调节免疫细胞、促进新生血管、支持血管生成和保护靶细胞免于凋亡。然而，尽管发现的历史相对较长，MSC和基于MSC的产品的生产仍然是一个重大挑战。对于细胞疗法，根据输注的方式和输注频率的不同，必须获得数百万个细胞才能达到最大的临床疗效。而为了生产临床相关大小的组织工程结构，必须获得数亿个细胞。这意味着MSC必须首先在体外扩增。因此，有效且经济地扩增MSC对于其在细胞治疗和组织工程中的应用具有至关重要的作用。",{"cn":456,"en":463,"jp":464,"kr":465},627,1286,982,531,1743597031,1743647203,{"id":150,"cateId":442,"catePath":9,"title":470,"lang":11,"path":471,"mainPic":472,"remark":9,"content":473,"otherLangId":475,"otherLangPath":20,"otherLangTitle":20,"cateName":9,"status":21,"sort":21,"pv":394,"statusText":9,"address":9,"city":9,"positionType":9,"recruitType":9,"employmentType":9,"createTime":479,"updateTime":480,"startTime":21,"stopTime":21,"breadCrumbs":20},"CAR-T 细胞治疗","car-t-xi-bao-zhi-liao","/dnweb/ca93a91250c8f9d91a89dab3f29acd80.png",{"desc":474,"pics":472,"url":9},"嵌合抗原受体 (CAR) 是一种独特的受体，旨在靶向特定肿瘤抗原，以对 T 淋巴细胞进行功能重编程。由于 T 淋巴细胞通过基因工程表达这些人工受体以靶向肿瘤细胞，因此这种疗法可称为免疫疗法、基因疗法或肿瘤疗法。",{"cn":150,"en":476,"jp":477,"kr":478},280,1100,796,1741094768,1743597323,[482,495],{"id":483,"cateId":400,"catePath":9,"title":484,"lang":11,"path":485,"mainPic":486,"remark":9,"content":487,"otherLangId":489,"otherLangPath":20,"otherLangTitle":20,"cateName":9,"status":21,"sort":21,"pv":493,"statusText":9,"address":9,"city":9,"positionType":9,"recruitType":9,"employmentType":9,"createTime":494,"updateTime":494,"startTime":21,"stopTime":21,"breadCrumbs":20},733,"重组胶原蛋白","zhong-zu-jiao-yuan-dan-bai","/dnweb/441e87466b5e127f261df62aee2561af.png",{"desc":488,"pics":486,"url":9},"胶原蛋白作为细胞外基质的核心成分，广泛存在于皮肤、骨骼、肌腱等多种组织中，不仅维持着细胞与组织的结构和功能，还在组织修复、创伤愈合等过程中发挥着不可替代的作用。根据其氨基酸排列和链组合方式的不同，胶原蛋白可以形成多种高级结构，目前已知人体中至少存在28种不同类型，每一种都具有独特的功能和生物学特性。正因如此，胶原蛋白被广泛应用于医疗健康和美妆护肤领域，比如用于皮肤再生、骨骼修复、肌腱愈合、止血、组织工程支架，以及药物缓释等场景。",{"cn":483,"en":490,"jp":491,"kr":492},734,1333,1029,590,1749446096,{"id":496,"cateId":400,"catePath":9,"title":497,"lang":11,"path":498,"mainPic":499,"remark":9,"content":500,"otherLangId":502,"otherLangPath":20,"otherLangTitle":20,"cateName":9,"status":21,"sort":21,"pv":456,"statusText":9,"address":9,"city":9,"positionType":9,"recruitType":9,"employmentType":9,"createTime":506,"updateTime":507,"startTime":21,"stopTime":21,"breadCrumbs":20},55,"胰岛素","yi-dao-su","/dnweb/6673070aca3243e6fd765b76fa014f74.png",{"desc":501,"pics":499,"url":9},"糖尿病是一种全球性疾病，有超过 4.2 亿人（世界人口的 6%）患有糖尿病，预计到 2030 年这一数字将激增至 5 亿，到 2045 年将激增至 7 亿。全世界糖尿病患者人数的增加将导致对胰岛素的需求随之增加。此外，对负担得起的胰岛素的需求未得到满足，尤其是在低收入和中等收入国家。许多完全依赖胰岛素生存的 1 型糖尿病患者无法获得胰岛素。",{"cn":496,"en":503,"jp":504,"kr":505},281,1101,797,1741416493,1743247660,[509,522,535,548,561],{"id":510,"cateId":387,"catePath":9,"title":511,"lang":11,"path":512,"mainPic":513,"remark":9,"content":514,"otherLangId":516,"otherLangPath":20,"otherLangTitle":20,"cateName":9,"status":21,"sort":21,"pv":520,"statusText":9,"address":9,"city":9,"positionType":9,"recruitType":9,"employmentType":9,"createTime":521,"updateTime":521,"startTime":21,"stopTime":21,"breadCrumbs":20},1596,"从配方到制造：解析细胞培养基可变性的来源与控制策略","cong-pei-fang-dao-zhi-zao-jie-xi-xi-bao-pei-yang-ji-ke-bian-xing-de-lai-yuan-yu-kong-zhi-ce-lve","/dnweb/63d7afab8991640150d39d50e7c86459.png",{"desc":515,"pics":513,"url":9},"在生物制药生产中，原材料被公认为影响工艺稳定性和产品一致性的关键可变来源，其中细胞培养基尤为重要。培养基组成及其批次差异不仅会影响细胞的生长速率、活力和生产力，还可能引发治疗性蛋白关键质量属性的变化。随着生物制药产业规模的持续扩大，以及高密度培养和连续生产工艺的逐步引入，行业对培养基配方稳定性、制造一致性及供应链可控性的要求显著提高。本文将从培养基配方演进的历史视角出发，简单介绍含血清体系向无血清及化学成分限定培养基的发展过程，并讨论液体与粉末培养基的制造机制。同时，介绍用于培养基表征与可变性根本原因分析的主要分析与监测手段，为理解和控制培养基可变性提供系统性参考。",{"cn":510,"en":517,"jp":518,"kr":519},1597,1599,1598,10,1767916874,{"id":523,"cateId":387,"catePath":9,"title":524,"lang":11,"path":525,"mainPic":526,"remark":9,"content":527,"otherLangId":529,"otherLangPath":20,"otherLangTitle":20,"cateName":9,"status":21,"sort":21,"pv":533,"statusText":9,"address":9,"city":9,"positionType":9,"recruitType":9,"employmentType":9,"createTime":534,"updateTime":534,"startTime":21,"stopTime":21,"breadCrumbs":20},1515,"抗体-脂质纳米颗粒（Ab-LNPs）：靶向递送的前沿技术与开发策略","kang-ti-zhi-zhi-na-mi-ke-li-ab-lnps-ba-xiang-di-song-de-qian-yan-ji-shu-yu-kai-fa-ce-lve","/dnweb/bb55c19e4387497197475bfc3e2b79a9.png",{"desc":528,"pics":526,"url":9},"近年来，随着纳米医学的快速发展，脂质纳米颗粒（Lipid Nanoparticles, LNP）已成为核酸药物和疫苗递送的核心平台，其中 COVID-19 mRNA 疫苗的成功更凸显了该技术的巨大潜力。在此基础上，抗体靶向脂质纳米颗粒（Antibody–Lipid Nanoparticles, Ab-LNP）作为一种融合抗体特异性识别与 LNP 高效递送能力的创新系统，正逐渐成为精准医疗的重要方向。与传统非靶向 LNP 相比，Ab-LNP 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